IgG Antibody to hepatitis D virus(HDV-IgG) ELISA Kit is anenzyme-linked immunosorbent assay (ELISA) for qualitative determination ofIgG antibodies to hepatitis D virus (HDV) in human serum or plasma. It isintended for use in clinical laboratories for diagnosis and management ofpatients related to infection with HDV.
Principle Of TheTest
IgG Antibody to hepatitis D virus(HDV-IgG) ELISA Kit is based onsolid phase, two-step incubation indirect ELISA method. Polystyrene microwellstrips are pre-coated with recombinant HDV antigens. During the firstincubation step, anti-HDV specific antibodies, if present, will be bound tothe solid phase pre-coated HDV antigens. The wells are washed to removeunbound serum proteins, and anti-human IgG antibodies (anti-IgG) conjugatedto the enzyme horseradish peroxidase (HRP-Conjugate) are added. During thesecond incubation step, these HRP-conjugated antibodies will be bound to anyantigen-antibody(IgG) complexes previously formed and the unbound HRP-conjugateis then removed by washing. Chromogen solutions containingTetramethylbenzidine (TMB) and urea peroxide are added to the wells and inpresence of the antigen-antibody-anti-IgG (HRP) immunocomplex, the colorlessChromogens are hydrolyzed by the bound HRP conjugate to a blue-coloredproduct. The blue color turns yellow after stopping the reaction withsulfuric acid. The amount of color intensity can be measured and it isproportional to the amount of antibody captured in the wells, and to theamount of antibody in the sample respectively. Wells containing samplesnegative for HDV IgG remain colorless.
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