Anti-Hepatitis G Virus(Anti-HGV) ELISA Kit recognizes the NS5 polyprotein of HGV, also known as GBV-C/HBV.
Immunogen
Peptide corresponding to amino acids 2268-2276 of the NS5 non-structural polyprotein of Hepatitis G Virus (HGV).
HGV ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of antibodies to hepatitis G virus (anti-HGV) in human serum or plasma. It is intended for use in clinical laboratories for diagnosis and management of patients related to infection with hepatitis G virus.
HGV ELISA kit employs solid phase, indirect ELISA method for detection of antibodies to HGV in two-step incubation procedure. Polystyrene microwell strip are pre-coated with recombinant, highly immunoreactive antigens corresponding to the structural regions of HGV. During the first incubation step, anti-HGV specific antibodies, if present, will be bound to the solid phase coated HGV antigens. The wells are washed to remove unbound materials and rabbit anti-human IgG antibodies (anti-IgG) conjugated to horseradish peroxidase (HRP-Conjugate) are added. During the second incubation step, these labeled antibodies will be bound to any antigen-antibody(IgG) complexes previously formed and the unbound labeled antibodies are removed by washing. Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the antigen-antibody-anti-IgG (HRP) immunocomplex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for anti-HGV remain colorless.
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