What is Anti-Hepatitis G Virus(Anti-HGV) ELISA Kit?

Specificity
Anti-Hepatitis G Virus(Anti-HGV) ELISA Kit recognizes the NS5 polyprotein of HGV, also known as GBV-C/HBV.
Immunogen
Peptide corresponding to amino acids 2268-2276 of the NS5 non-structural polyprotein of Hepatitis G Virus (HGV).
HGV ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of antibodies to hepatitis G virus (anti-HGV) in human serum or plasma. It is intended for use in clinical laboratories for diagnosis and management of patients related to infection with hepatitis G virus.
HGV ELISA kit employs solid phase, indirect ELISA method for detection of antibodies to HGV in two-step incubation procedure. Polystyrene microwell strip are pre-coated with recombinant, highly immunoreactive antigens corresponding to the structural regions of HGV. During the first incubation step, anti-HGV specific antibodies, if present, will be bound to the solid phase coated HGV antigens. The wells are washed to remove unbound materials and rabbit anti-human IgG antibodies (anti-IgG) conjugated to horseradish peroxidase (HRP-Conjugate) are added. During the second incubation step, these labeled antibodies will be bound to any antigen-antibody(IgG) complexes previously formed and the unbound labeled antibodies are removed by washing. Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the antigen-antibody-anti-IgG (HRP) immunocomplex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for anti-HGV remain colorless.
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Where to find Anti-Hepatitis G Virus(Anti-HGV) ELISA Kit?

GB virus C (GBV-C), formerly known as hepatitis G virus (HGV), is a virus in the Flaviviridae family which has not yet been assigned to a genus, is known to infect humans, but is not known to cause human disease. There have been reports that HIV patients coinfected with GBV-C can survive longer than those without GBV-C, but the patients may be different in other ways. There is current active research into the virus' effects on the immune system in patients coinfected with GBV-C and HIV.
Hepatitis G Virus infection was originally suggested to be connected with fulminant hepatitis, but recent studies have failed to prove a connection between HGV and clinical illness. Some studies have suggested that in contrast to HCV, the liver is not the primary replication site for HGV. Where does HGV grow? Virus circulating in the bloodstream is difficult precipitate with antibody to immunoglobulins, but can precipitated with antibody to apolipoproteins. Since no hypervariable regions have yet been identified in the envelope proteins of HGV, the lipoprotein coat may help the virus evade immune surveillance and contribute to its persistence.
Human infection
The majority of immune-competent individuals appear to clear GBV-C viraemia within the first few years following infection and although the time interval between GBV-C infection and clearance of viraemia (detection of GBV-C RNA in plasma) is not known, infection may persist for decades in some individuals.
Approximately 2% of healthy US blood donors are viraemic with GBV-C, and up to 13% of blood donors have antibodies to E2 protein, indicating prior infection.
Parenteral, sexual and vertical transmission of GBV-C have all been documented, and because of shared modes of transmission, individuals infected with HIV are commonly co-infected with GBV-C. Among people with HIV infection, the prevalence of GBV-C viraemia ranges from 14 to 43%.
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